Hadassah-Hebrew University Medical Center 4 articles published in JoVE Biology A BW Reporter System for Studying Receptor-Ligand Interactions Shlomo Elias1,2, Shira Kahlon2, Alexandra Duev-Cohen2, Ofer Mandelboim2 1Department of Hematology, Hadassah - Hebrew University Medical Center, 2The Lautenberg Center for General and Tumor Immunology, Department of Immunology and Cancer Research, Institute for Medical Research Israel Canada (IMRIC), Hadassah - Hebrew University Medical Center This protocol describes how to establish a reporter system that can be used to identify and quantify receptor-ligand interactions. Immunology and Infection Isolation and Characterization of Neutrophils with Anti-Tumor Properties Ronit Vogt Sionov1, Simaan Assi1, Maya Gershkovitz1, Jitka Y. Sagiv1, Lola Polyansky1, Inbal Mishalian2, Zvi G. Fridlender2, Zvi Granot1 1Department of Developmental Biology and Cancer Research, Institute for Medical Research Israel-Canada, Hebrew University Medical School, 2Institute of Pulmonary Medicine, Hadassah-Hebrew University Medical Center Neutrophils play an important role not only in host defense against invading microorganisms, but are also involved in the immune surveillance of tumor cells. Here, we describe techniques related to the isolation of neutrophils with anti-tumor properties and methods for monitoring anti-tumor neutrophil function in vitro and in vivo. Medicine Dynamic Visual Tests to Identify and Quantify Visual Damage and Repair Following Demyelination in Optic Neuritis Patients Noa Raz1, Michal Hallak1, Tamir Ben-Hur1, Netta Levin1 1Department of Neurology, The Agnes Ginges Center for Human Neurogenetics, Hadassah Hebrew-University Medical Center Object From Motion (OFM) and Time-constrained stereo protocols are sensitive tools to identify monocular and binocular dynamic visual function deficits, which are uniquely affected in optic neuritis patients. Furthermore, these tests may be used as quantitative noninvasive tools to assess the extent of myelination along visual pathways. Immunology and Infection Isolation, Processing and Analysis of Murine Gingival Cells Gabriel Mizraji*1,2, Hadas Segev*1, Asaf Wilensky*2, Avi-Hai Hovav*1 1Institute of Dental Sciences, Hebrew University - Hadassah Medical Center, 2Department of Periodontology, Hebrew University - Hadassah Medical Center This study describes an efficient technique to isolate and process gingival tissues from the mouse oral cavity in order to produce a single-cell culture. The resulting cells can be further used for flow cytometry analysis and molecular studies.