IRCCS Istituti Clinici Scientifici Maugeri 2 articles published in JoVE Cancer Research Isolation of Primary Cancer-Associated Fibroblasts from a Syngeneic Murine Model of Breast Cancer for the Study of Targeted Nanoparticles Marta Truffi*1, Leopoldo Sitia*2, Marta Sevieri2, Arianna Bonizzi2, Maria Antonietta Rizzuto3, Serena Mazzucchelli2, Fabio Corsi1,2 1Istituti Clinici Scientifici Maugeri IRCCS, 2Dipartimento di Scienze Biomediche e Cliniche “L. Sacco”, Università di Milano, 3Dipartimento di Biotecnologie e Bioscienze, Università di Milano-Bicocca This paper aims to provide a protocol for the isolation and culture of primary cancer-associated fibroblasts from a syngeneic murine model of triple-negative breast cancer and their application for the preclinical study of novel nanoparticles designed to target the tumor microenvironment. Immunology and Infection A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC Gabriele Babini*1, Jacopo Morini*1, Sofia Barbieri1, Giorgio Baiocco1, Giovanni Battista Ivaldi2, Marco Liotta2, Paola Tabarelli de Fatis2, Andrea Ottolenghi1 1Dipartimento di Fisica, Università degli Studi di Pavia, 2IRCCS Istituti Clinici Scientifici Maugeri We present a protocol to investigate the crosstalk between X-ray-irradiated Caco-2 and peripheral blood mononuclear cells (PBMC). The protocol starts with Caco-2 irradiation and set-up of the co-culture with PBMC; subsequently, trans-epithelial electrical resistance is measured regularly over 48 h and western blot performed in both Caco-2 and PBMC.