Polytechnic of Leiria 1 article published in JoVE Biology Valorization of the Red Seaweed Gracilaria gracilis Through a Biorefinery Approach Alice Martins1, Filipa R Pinto1, Sónia Barroso1, Tatiana Pereira1, Teresa Mouga1, Clélia Afonso1, Marta V Freitas1,2, Susete Pinteus1, Rui Pedrosa1, Maria M Gil1 1MARE-Marine and Environmental Sciences Centre/ARNET-Aquatic Research Network, ESTM, Polytechnic University of Leiria, 2MARE-Marine and Environmental Sciences Centre/ARNET-Aquatic Research Network, Department of Life Sciences, University of Coimbra Here, we describe several protocols aiming at an integrated valorization of Gracilaria gracilis: wild species harvesting, in-house growth, and extraction of bioactive ingredients. The extracts' antioxidant, antimicrobial, and cytotoxic effects are evaluated, along with the nutritional and stability assessment of food enriched with whole seaweed biomass and pigments.