Life Science Methods BV 1 article published in JoVE Immunology and Infection Establishment and Optimization of a High Throughput Setup to Study Staphylococcus epidermidis and Mycobacterium marinum Infection as a Model for Drug Discovery Wouter J. Veneman*1, Rubén Marín-Juez*2, Jan de Sonneville3, Anita Ordas1, Susanne Jong-Raadsen2, Annemarie H. Meijer1, Herman P. Spaink1 1Institute of Biology, Leiden University, 2ZF-screens BV, 3Life Science Methods BV This video article describes the high throughput pipeline that has been successfully established to infect and analyze large numbers of zebrafish embryos providing a new powerful tool for compound testing and drug discovery using a whole animal vertebrate organism.