Stanford Medical School 1 article published in JoVE Medicine An Affordable HIV-1 Drug Resistance Monitoring Method for Resource Limited Settings Justen Manasa1, Siva Danaviah1, Sureshnee Pillay1, Prevashinee Padayachee1, Hloniphile Mthiyane1, Charity Mkhize1, Richard John Lessells1, Christopher Seebregts2, Tobias F. Rinke de Wit3, Johannes Viljoen1, David Katzenstein4, Tulio De Oliveira1 1Africa Centre for Health and Population Studies, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa, 2Unit D11, Jembi Health Systems, 3Academic Medical Center, Department of Global Health, Amsterdam Institute for Global Health and Development (AIGHD), University of Amsterdam, 4Division of Infectious Diseases and Geographic Medicine, Centre for AIDS Research, Stanford Medical School Drug resistance testing for HIV-1 infected individuals failing antiretroviral therapy (ART) can guide future therapies and improve treatment outcomes. Optimizing individual and population health outcomes in high HIV prevalence but resource-limited settings will ultimately require affordable and accessible drug resistance genotyping and interpretation methods.