Rosalind Franklin University of Medicine and Science View Institution's Website 5 articles published in JoVE Biology Photodiode-Based Optical Imaging for Recording Network Dynamics with Single-Neuron Resolution in Non-Transgenic Invertebrates Evan S. Hill*1,2, Jeffrey W. Brown*1,2, William N. Frost1,2 1Cell Biology and Anatomy, Chicago Medical School, Rosalind Franklin University of Medicine and Science, 2Center for Brain Function and Repair, Rosalind Franklin University of Medicine and Science This protocol presents a method for imaging neuronal population activity with single-cell resolution in non-transgenic invertebrate species using absorbance voltage-sensitive dyes and a photodiode array. This approach enables a rapid workflow, wherein imaging and analysis can be pursued over the course of a single day. Medicine A Rat Model of Ventricular Fibrillation and Resuscitation by Conventional Closed-chest Technique Lorissa Lamoureux1, Jeejabai Radhakrishnan1, Raúl J. Gazmuri1 1Resuscitation Institute, Rosalind Franklin University of Medicine and Science This article describes a rat model of electrically-induced ventricular fibrillation and resuscitation by chest compression, ventilation, and delivery of electrical shocks that simulates an episode of sudden cardiac arrest and conventional cardiopulmonary resuscitation. The model enables gathering insights on the pathophysiology of cardiac arrest and exploration of new resuscitation strategies. Immunology and Infection Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets Stephanie Flaherty1, Joseph M. Reynolds1 1Department of Microbiology and Immunology, The Chicago Medical School, Rosalind Franklin University of Medicine and Science Naïve CD4+ T cells polarize to various subsets depending on the environment at the time of activation. The differentiation of naïve CD4+ T cells to various effector subsets can be achieved in vitro through the addition of T cell receptor stimuli and specific cytokine signals. Biology An Assay for Lateral Line Regeneration in Adult Zebrafish Gina C. Pisano*1, Samantha M. Mason*2, Nyembezi Dhliwayo1, Robert V. Intine1, Michael P. Sarras, Jr.2 1Department of Biomedical Sciences, Dr. William M Scholl College of Podiatric Medicine, Rosalind Franklin University of Medicine and Science, 2Department of Cell Biology and Anatomy, Rosalind Franklin University of Medicine and Science Because many zebrafish models of neurological and non-neurological diseases are studied in the adult fish rather than the embryo/larvae, we developed a quantitative lateral line regenerative assay that can be applied to adult zebrafish disease models. The assay involved resolution at the 1) neuromast and 2) individual hair cell levels. Medicine A Zebrafish Model of Diabetes Mellitus and Metabolic Memory Robert V. Intine1, Ansgar S. Olsen1, Michael P. Sarras Jr.2 1Dr. William M. Scholl College of Podiatric Medicine, Rosalind Franklin University of Medicine and Science, 2Chicago Medical School, Rosalind Franklin University of Medicine and Science Metabolic memory is the phenomenon by which diabetic complications persist and progress unimpeded even after euglycemia is achieved pharmaceutically. Here we describe a diabetes mellitus zebrafish model which is unique in that it allows for the examination of the mitotically transmissible epigenetic components of metabolic memory in vivo.