Jacobs Technology Inc. 1 article published in JoVE Engineering Determining Viral Disinfection Efficacy of Hot Water Laundering Anne Mikelonis*1, John Archer*1, Barbara Wyrzykowska-Ceradini2, Eric Morris3, Jonathan Sawyer2, Timothy Chamberlain2, Ahmed Abdel-Hady2, Mariela Monge4, Abderrahmane Touati2 1Office of Research and Development, Center for Environmental Solutions and Emergency Response, Homeland Security and Materials Management Division, U.S. Environmental Protection Agency, 2Jacobs Technology Inc., 3Science Systems and Applications Inc., 4CSS Inc. In response to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, a laboratory protocol was developed to test the viral disinfection efficacy of hot water laundering of cloth face coverings, cotton scrubs, and denim pants. The Phi6 virus (bacteriophage) was used as the organism to test disinfection efficacy.