University of Nebraska Medical Centre 1 article published in JoVE Immunology and Infection Probiotic Studies in Neonatal Mice Using Gavage Freddy Francis1, Natallia Varankovich2, Byron Brook1, Nelly Amenyogbe1, Rym Ben-Othman2, Bing Cai2, Danny Harbeson1, Aaron C. Liu1, Ben Dai2, Shelly McErlane4, Kris Andrews4, Tobias R. Kollmann*1,2, Pinaki Panigrahi*3 1Department of Experimental Medicine, University of British Columbia, 2Department of Pediatrics, University of British Columbia, 3Department of Epidemiology and Pediatrics, University of Nebraska Medical Centre, 4Animal Care Services, University of British Columbia This study details the process of gavaging precise amounts of probiotics to neonatal mice. The experimental set-up was optimized to include but is not limited to probiotic dosing, methods of administration, and quantification of bacteria in the intestines.