Center for Conservation and Research of Endangered Wildlife 1 article published in JoVE Biology Reproductive Techniques for Ovarian Monitoring and Control in Amphibians Natalie E. Calatayud1,2, Norin Chai3, Nicole R. Gardner2, Michelle J. Curtis2, Monica A. Stoops4 1Reproductive Sciences, San Diego Zoo Institute for Conservation Research, 2Recovery Ecology, San Diego Zoo Institute for Conservation Research, 3 The study of amphibian biology provides valuable information on the reproductive, physiological, embryological and developmental processes that drive organisms of many taxonomic groups. Here, we present a comprehensive guide on different methodologies that can be used to study ovarian control and monitoring in amphibians.