UT Southwestern Medical Center 3 articles published in JoVE Bioengineering Light-Sheet Imaging to Reveal Cardiac Structure in Rodent Hearts Milad Almasian1, Alireza Saberigarakani1, Xinyuan Zhang1, Brian Lee1, Yichen Ding1,2,3 1Department of Bioengineering, The University of Texas at Dallas, 2Center for Imaging and Surgical Innovation, The University of Texas at Dallas, 3Hamon Center for Regenerative Science and Medicine, UT Southwestern Medical Center The protocol utilizes advanced light-sheet microscopy along with adapted tissue clearing methods to investigate intricate cardiac structures in rodent hearts, holding great potential for the understanding of cardiac morphogenesis and remodeling. Bioengineering 4D Light-sheet Imaging of Zebrafish Cardiac Contraction Xinyuan Zhang1, Alireza Saberigarakani1, Milad Almasian1, Sohail Hassan1, Manasa Nekkanti1, Yichen Ding1,2,3 1Department of Bioengineering, The University of Texas at Dallas, 2Center for Imaging and Surgical Innovation, The University of Texas at Dallas, 3Hamon Center for Regenerative Science and Medicine, UT Southwestern Medical Center This protocol utilizes light-sheet imaging to investigate cardiac contractile function in zebrafish larvae and gain insights into cardiac mechanics through cell tracking and interactive analysis. Medicine Murine Orchiectomy and Ovariectomy to Reduce Sex Hormone Production Ashley A. Rowe1, Yacine Issioui1, Blessing Johnny1, Katherine J. Wert1,2,3,4 1Department of Ophthalmology, UT Southwestern Medical Center, 2Department of Molecular Biology, UT Southwestern Medical Center, 3Hamon Center for Regenerative Science and Medicine, UT Southwestern Medical Center, 4Peter O'Donnell Jr. Brain Institute, UT Southwestern Medical Center This manuscript describes a consistent way to quickly perform survival rodent orchiectomies and ovariectomies.
