University of North Texas View Institution's Website 6 articles published in JoVE Behavior Gathering Self-Initiated Rat Behavioral Data to Characterize Post-Stroke Deficits Jared Armshaw*1, Grayson Butcher*1, April Becker1 1Department of Behavior Analysis, College of Health and Public Service, University of North Texas A system for acquiring data from self-initiated individual behavior sessions within a social colony cage setting is presented. The efficacy of this system is demonstrated using an automated skilled reach assessment, enabling the characterization of post-stroke motor impairments, potential behavioral alterations related to motivation, circadian variations, and other innovative dependent variables. Engineering Indirect Fabrication of Lattice Metals with Thin Sections Using Centrifugal Casting Jiwon Mun1, Jaehyung Ju1, James Thurman2 1Department of Mechanical and Energy Engineering, University of North Texas, 2Department of Studio Art, University of North Texas An indirect additive manufacturing method combining a 3D printing of polymers with a centrifugal casting is outlined for manufacturing 3D octet truss metals (Al and Cu alloys) having a unit cell length of 5 mm with a wall thickness of 0.5 mm. Engineering Atomically Traceable Nanostructure Fabrication Josh B. Ballard1, Don D. Dick2, Stephen J. McDonnell3, Maia Bischof4, Joseph Fu5, James H. G. Owen1, William R. Owen1, Justin D. Alexander1, David L. Jaeger4, Pradeep Namboodiri5, Ehud Fuchs1, Yves J. Chabal3, Robert M. Wallace3, Richard Reidy4, Richard M. Silver5, John N. Randall1, James Von Ehr1 1Zyvex Labs, 2Department of Physics, University of Texas at Dallas, 3Department of Materials Science and Engineering, University of Texas at Dallas, 4Materials Science and Engineering, University of North Texas, 5National Institute of Standards and Technology We report a protocol for combining the atomic metrology of the Scanning Tunneling Microscope for surface patterning with selective Atomic Layer Deposition and Reactive Ion Etching. Using a robust process involving numerous atmospheric exposures and transport, 3D nanostructures with atomic metrology are fabricated. Immunology and Infection Image-based Flow Cytometry Technique to Evaluate Changes in Granulocyte Function In Vitro Brian K. McFarlin1, Adam S. Venable1, Eric A. Prado1, Andrea L. Henning1, Randall R. Williams1 1Applied Physiology Laboratory, University of North Texas This method demonstrates a technique for assessing granulocyte function by simultaneously measuring phagocytosis of bacteria and oxidative burst. Image-based flow cytometry allowed for the identification of three distinct subsets of activated granulocytes that all differed in their relative functional capacity. Biology Use of Time Lapse Microscopy to Visualize Anoxia-induced Suspended Animation in C. elegans Embryos Anastacia M. Garcia1, Mary L. Ladage1, Pamela A. Padilla1 1Department of Biological Sciences, University of North Texas Described here is an in vivo technique to image sub-cellular structures in animals exposed to anoxia using a gas flow through microincubation chamber in conjunction with a spinning disc confocal microscope. This method is straightforward and flexible enough to suit a variety of experimental parameters and model systems. Bioengineering Demonstrating the Uses of the Novel Gravitational Force Spectrometer to Stretch and Measure Fibrous Proteins James W. Dunn1, Douglas D. Root1 1Department of Biological Sciences, University of North Texas This is a step-by step guide showing the purpose, operation, and representative results from the novel gravitational force spectrometer.
