Eckholm Farm 1 article published in JoVE Biology Methods for Comparing Nutrients in Beebread Made by Africanized and European Honey Bees and the Effects on Hemolymph Protein Titers Gloria Degrandi-Hoffman1, Bruce Eckholm2, Ming Huang3 1Carl Hayden Bee Research Center, USDA-ARS, 2Coupeville, WA, USA, 3Ecotoxicology Division, Eurofins Agroscience Services, Inc. Here are methods to quantify nutrients in pollen before and after its conversion to beebread by two subspecies of honeybees. We describe techniques to measure beebread consumption and resulting protein titers in both subspecies.