Marquette University 4 articles published in JoVE Medicine Evaluation of Vascular Control Mechanisms Utilizing Video Microscopy of Isolated Resistance Arteries of Rats Kathleen M. Lukaszewicz1, Matthew J. Durand2, Jessica R.C. Priestley3, James R. Schmidt4, L. Adrienne Allen5, Aron M. Geurts3, Julian H. Lombard3 1Department of Physical Therapy, Marquette University, 2Medical College of Wisconsin, 3Department of Physiology, Medical College of Wisconsin, 4Graduate Programs of Nurse Anesthesia, Texas Wesleyan University, 5Office of Research, Medical College of Wisconsin This manuscript describes in vitro video microscopy protocols for evaluating vascular function in rat cerebral resistance arteries. The manuscript also describes techniques for evaluating microvessel density with fluorescently labeled lectin and tissue perfusion using Laser Doppler Flowmetry. Engineering Thermal Measurement Techniques in Analytical Microfluidic Devices Benyamin Davaji1, Chung Hoon Lee1 1Nanoscale Devices Laboratory, Marquette University Here, we present three protocols for thermal measurements in microfluidic devices. Neuroscience Diffusion Imaging in the Rat Cervical Spinal Cord Elizabeth Zakszewski1, Brian Schmit2, Shekar Kurpad1, Matthew D. Budde1 1Department of Neurosurgery, Medical College of Wisconsin, 2Department of Biomedical Engineering, Marquette University The goal of this protocol is to obtain high-quality diffusion weighted magnetic resonance imaging (DWI) of the rat spinal cord for noninvasive characterization of tissue microstructure. This protocol describes optimizations of the MRI sequence, radiofrequency coil, and analysis methods to enable DWI images free from artifacts. Behavior An Alternative to the Traditional Cold Pressor Test: The Cold Pressor Arm Wrap Anthony John Porcelli1 1Department of Psychology, Marquette University The cold pressor test is a classic stress induction task used in a wide range of research examining the relationship between stress and cognition, emotion, and behavior. In this article, a practical and versatile alternative to the traditional cold pressor test is presented: the cold pressor arm wrap.