USDA-ARS Arthropod-Borne Animal Diseases Research Unit, Center for Grain and Animal Health Research 1 article published in JoVE Behavior Key Elements of Photo Attraction Bioassay for Insect Studies or Monitoring Programs Lee W. Cohnstaedt1, Jordan C. Disberger1, Eric Paulsen2, Adrian J. Duehl3 1Arthropod-Borne Animal Diseases Research Unit, Center for Grain and Animal Health Research, Agricultural Research Service, United States Department of Agriculture, 2Center for Medical, Agricultural, and Veterinary Entomology, Agricultural Research Service, United States Department of Agriculture, 3Bayer Photo-attraction bioassay arenas are used to determine the optimal light color(s) to maximize insect attraction; however bioassays and methods are specific to target insect behaviors and habitats. Customizable equipment and modifications are explained for nocturnal or diurnal and terrestrial or aerial insects.