The Forsyth Institute 1 article published in JoVE Biology Establishing Stable Binary Cultures of Symbiotic Saccharibacteria from the Oral Cavity Andrew J. Collins*1,2, Pallavi P. Murugkar*1,2, Floyd E. Dewhirst1,2 1The Forsyth Institute, 2Harvard School of Dental Medicine We demonstrate a method for isolating difficult-to-grow members of the novel bacterial phylum, Saccharibacteria, by filtering dental plaque and co-culturing with host bacteria.