State University of Campinas (UNICAMP) 1 article published in JoVE Biology Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants Laís Soêmis Sisti1, Matheus Pena-Passos1, Juliana Lishcka Sampaio Mayer1 1LabPlaM - Mycoheterotrophic Plants Research Lab, Department of Plant Biology, State University of Campinas (UNICAMP) The current article aims to provide detailed and adequate protocols for the isolation of plant-associated endophytic fungi, long-term preservation of isolates, morphological characterization, and total DNA extraction for subsequent molecular identification and metagenomic analyses.