JOVE JOURNAL

Biochimica

Showing 601 - 612 in 724 videos.

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Journal (Biochemistry)

Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions

Robert W. Harkness V, Philip E. Johnson, Anthony K. Mittermaier

¹Department of Chemistry,McGill University, ²Department of Chemistry,York University

Journal (Biochemistry)

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Anthony A. High, Haiyan Tan, Vishwajeeth R. Pagala, Mingming Niu³, Ji-Hoon Cho, Xusheng Wang, Bing Bai, Junmin Peng²

¹St. Jude Proteomics Facility,St. Jude Children's Research Hospital, ²Departments of Structural Biology and Developmental Neurobiology,St. Jude Children's Research Hospital, ³Heilongjiang University of Chinese Medicine

Journal (Biochemistry)

Dissection of Human Retina and RPE-Choroid for Proteomic Analysis

Thiago Cabral*^1,2,7,8, Marcus A. Toral*^3,4, Gabriel Velez⁴, James E. DiCarlo², Anuradha M. Gore, MaryAnn Mahajan, Stephen H. Tsang², Alexander G. Bassuk⁶, Vinit B. Mahajan⁹

¹Barbara & Donald Jonas Stem Cell Laboratory, and Bernard & Shirlee Brown Glaucoma Laboratory, Department of Pathology & Cell Biology, Institute of Human Nutrition, College of Physicians and Surgeons,Columbia University, ²Edward S. Harkness Eye Institute,New York-Presbyterian Hospital, ³Omics Laboratory, Byers Eye Institute, Department of Ophthalmology,Stanford University, ⁴Medical Scientist Training Program,University of Iowa, ⁵Department of Pediatrics,University of Iowa, ⁶Department of Neurology,University of Iowa, ⁷Department of Ophthalmology,Federal University of Sao Paulo (UNIFESP), ⁸Department of Ophthalmology,Federal University of EspÍrito Santo (UFES), ⁹Palo Alto Veterans Administration, Palo Alto, CA

Journal (Biochemistry)

Improved Protocol for Chromatin Immunoprecipitation from Mouse Skeletal Muscle

Shilpy Joshi, Vanessa Ueberschlag-Pitiot, Daniel Metzger, Irwin Davidson

¹Department of Functional Genomics and Cancer,Institut de Génétique et de Biologie Moléculaire et Cellulaire

Journal (Biochemistry)

Enrichment of Detergent-insoluble Protein Aggregates from Human Postmortem Brain

Ian Diner, Tram Nguyen, Nicholas T. Seyfried

¹Department of Biochemistry,Emory School of Medicine

Journal (Biochemistry)

Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging

Sofie Nyström, Marcus Bäck, K. Peter R. Nilsson, Per Hammarström

¹IFM-Department of Chemistry,Linköping University

Journal (Biochemistry)

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE

Venkataramana R. Pidatala*^1,2, Amir Mahboubi*^1,2, Jenny C. Mortimer²

¹Joint BioEnergy Institute, ²Environmental and Systems Biology, BioSciences Division,Lawrence Berkeley National Laboratory

Journal (Biochemistry)

Assay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells

Md Nazmul Huda², Erdenedolgor Erdene-Ochir², Cheol-Ho Pan²

¹Systems Biotechnology Research Center,KIST Gangneung Institute of Natural Products, ²Division of Bio-Medical Science & Technology, KIST School,Korea University of Science and Technology

Journal (Biochemistry)

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

Eliza L. Lewis, Aaron M. Leconte

¹Department of Chemistry,W.M. Keck Science Department of Claremont Mckenna, Pitzer, and Scripps College

Journal (Biochemistry)

Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092

Simone Culurgioni², Minzhe Tang², David R. Hall, Martin A. Walsh²

¹Diamond Light Source,Harwell Science & Innovation Campus, ²Research Complex at Harwell,Harwell Science & Innovation Campus

Journal (Biochemistry)

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA

Kan Xiong², Paul C. Blainey²

¹Broad Institute,Massachusetts Institute of Technology and Harvard Medical School, ²Dept. of Biological Engineering,Massachusetts Institute of Technology

Journal (Biochemistry)

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

Robert Warneford-Thomson⁴, Chongsheng He², Simone Sidoli³, Benjamin A. Garcia³, Roberto Bonasio²

¹Epigenetics Institute,University of Pennsylvania Perelman School of Medicine, ²Department of Cell and Developmental Biology,University of Pennsylvania Perelman School of Medicine, ³Department of Biochemistry and Biophysics,University of Pennsylvania Perelman School of Medicine, ⁴Graduate Group in Biochemistry and Biophysics,University of Pennsylvania Perelman School of Medicine

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