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Fresh Primary Tumor Tissue Processing to Establish Tumor Organoids and Primary Fibroblasts

¹Molecular Epidemiology and Predictive Tumor Markers Group, Area 3,Ramón y Cajal Health Research Institute (IRYCIS), ²The Biomedical Research Network in Cancer (CIBERONC), ³Biobank and Biomodels Platform (PT20/0045), ISCIII research and development platforms in biomedicine and health sciences, BioBank Hospital Ramón y Cajal-IRYCIS, Spanish National Biobanks Network (ISCIII Biobank Register No. B.0000678),Ramón y Cajal Health Research Institute (IRYCIS), ⁴Faculty of Medicine,University of Alcalá de Henares, ⁵Institute of Tissue Medicine and Pathology,University of Bern, ⁶Department of Molecular Oncology, Cancer Research Institute,Biomedical Research Center of the Slovak Academy of Sciences, ⁷Experimental Oncology, Molecular Oncology Program,Centro Nacional de Investigaciones Oncológicas (CNIO), ⁸Department of Surgical Oncology, National Cancer Institute,Slovak Medical University, ⁹Pancreatic and Biliopancreatic Surgery Unit,Hospital Universitario Ramón y Cajal, ¹⁰Department of Pathology,Hospital Universitario Ramón y Cajal, ¹¹Department of Radiation Oncology,Hospital Universitario Ramón y Cajal, ¹²Department of Cancer,Instituto de Investigaciones Biomédicas “Alberto Sols” (IIBM), ¹³Cancer Stem Cell and Fibroinflammatory Group, Chronic Diseases and Cancer, Area 3,IRYCIS

Journal (Cancer Research)

Shear Assay Protocol for the Determination of Single-Cell Material Properties

Luke J. Holen*¹, Killian Onwudiwe*¹, Julian Najera, Maksym Zarodniuk, John D. Obayemi, Winston O. Soboyejo, Meenal Datta

¹Department of Aerospace and Mechanical Engineering,University of Notre Dame, ²Departments of Mechanical and Biomedical Engineering,Worcester Polytechnic Institute

Journal (Cancer Research)

Microfluidic Shear Apparatus Setup for Single-Cell Fluid Shear Assay

Luke J. Holen*¹, Killian Onwudiwe*¹, Julian Najera, Maksym Zarodniuk, John D. Obayemi, Winston O. Soboyejo, Meenal Datta

¹Department of Aerospace and Mechanical Engineering,University of Notre Dame, ²Departments of Mechanical and Biomedical Engineering,Worcester Polytechnic Institute

Journal (Cancer Research)

Performing Single-Cell Shear Assay and Imaging

Luke J. Holen*¹, Killian Onwudiwe*¹, Julian Najera, Maksym Zarodniuk, John D. Obayemi, Winston O. Soboyejo, Meenal Datta

¹Department of Aerospace and Mechanical Engineering,University of Notre Dame, ²Departments of Mechanical and Biomedical Engineering,Worcester Polytechnic Institute

Journal (Cancer Research)

Processing the Single-Cell Shear Assay Imaging Data

Luke J. Holen*¹, Killian Onwudiwe*¹, Julian Najera, Maksym Zarodniuk, John D. Obayemi, Winston O. Soboyejo, Meenal Datta

¹Department of Aerospace and Mechanical Engineering,University of Notre Dame, ²Departments of Mechanical and Biomedical Engineering,Worcester Polytechnic Institute

Journal (Cancer Research)

Tracking Bispecific Antibody-Induced T Cell Trafficking Using Luciferase-Transduced Human T Cells

Madelyn Espinosa-Cotton, Hong-Fen Guo, Nai-Kong V. Cheung

¹Department of Pediatrics,Memorial Sloan Kettering Cancer Center

Journal (Cancer Research)

Establishment of Zebrafish Patient-Derived Xenografts from Pancreatic Cancer for Chemosensitivity Testing

Alice Usai, Gregorio Di Franco, Chiara Gabellini, Luca Morelli, Vittoria Raffa

¹Department of Biology,University of Pisa, ²General Surgery Unit, Department of Translational Research and New Technologies in Medicine and Surgery,University of Pisa

Journal (Cancer Research)

Somatic Genome-Engineered Mouse Models Using In Vivo Microinjection and Electroporation

Keerthana Harwalkar², Nobuko Yamanaka³, Yojiro Yamanaka^2,3

¹Rosalind and Morris Goodman Cancer Research Institute,McGill University, ²Department of Human Genetics,McGill University, ³McGill Integrated Core for Animal Modeling (MICAM),McGill University

Journal (Cancer Research)

A2780 Cells Genomic DNA Isolation and Analysis

Meghna Jain*¹, Shrey Madeka*¹, Ekta Khattar

¹Sunandan Divatia School of Science,SVKM's NMIMS Deemed-to-be-University

Journal (Cancer Research)

Southern Blotting, Hybridization, and Chemiluminescence Detection of Genomic DNA Isolated from A2780 Cells

Meghna Jain*¹, Shrey Madeka*¹, Ekta Khattar

¹Sunandan Divatia School of Science,SVKM's NMIMS Deemed-to-be-University

Journal (Cancer Research)

Optimization of Performance Parameters of the TAGGG Telomere Length Assay

Meghna Jain*¹, Shrey Madeka*¹, Ekta Khattar

¹Sunandan Divatia School of Science,SVKM's NMIMS Deemed-to-be-University

Journal (Cancer Research)

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Saxon Rodriguez*¹, Baohua Sun*¹, Salome McAllen, Mei Jiang, Edwin Roger Parra

¹Department of Translational Molecular Pathology,The University of Texas MD Anderson Cancer Center

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