All Protocols and Video Articles for scientific research | JoVE

Showing 325 - 336 of 477 videos for "Reconstitution"

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Journal (Biology)

Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles

Harald Lahm*¹, Stefanie A. Doppler*¹, Martina Dreßen, Klaudia Adamczyk, Marcus-André Deutsch, Hanna Ulrich, Matthias Schiemann³, Rüdiger Lange⁴, Markus Krane⁴

¹Department of Cardiovascular Surgery, German Heart Center Munich,Technische Universität München, ²Institute for Medical Microbiology, Immunology, and Hygiene,Technische Universität München, ³Clinical Cooperation Groups: "Antigen-specific Immunotherapy" and "Immune Monitoring", Helmholtz Center Munich (Neuhererg),Technische Universität München, ⁴DZHK (German Center for Cardiovascular Research) – Partner site Munich Heart Alliance

Journal (Bioengineering)

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation (BiFC-PALM)

Andrew Nickerson^2,3, Tao Huang^2,3, Li-Jung Lin^2,3, Xioalin Nan^2,3

¹Department of Biomedical Engineering,Oregon Health and Science University, ²Knight Cancer Institute,Oregon Health and Science University, ³OHSU Center for Spatial Systems Biomedicine,Oregon Health and Science University

Journal (Biology)

Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis

Alexandra Naba, Karl R. Clauser, Richard O. Hynes

¹Koch Institute for Integrative Cancer Research,Massachusetts Institute of Technology, ²Proteomics Platform,Broad Institute

Journal (Neuroscience)

Observation of the Ciliary Movement of Choroid Plexus Epithelial Cells Ex Vivo

Takafumi Inoue, Keishi Narita, Yuta Nonami, Hideki Nakamura, Sen Takeda

¹Department of Life Science and Medical Bioscience, Faculty of Science and Engineering,Waseda University, ²Department of Anatomy and Cell Biology, Interdisciplinary Graduate School of Medicine & Engineering,University of Yamanashi

Journal (Bioengineering)

Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect

Daniel S. Oh, Alia Koch, Sidney Eisig, Sahng Gyoon Kim, Yoon Hyuk Kim, Do-Gyoon Kim, Jae Hyuck Shim

¹Oral and Maxillofacial Surgery,Columbia University, ²Endodontics,Columbia University, ³Mechanical Engineering,Kyung Hee University, South Korea, ⁴Orthodontics,The Ohio State University, ⁵Pathology,Weill Cornell Medical College

Journal (Immunology and Infection)

Automated Cell Enrichment of Cytomegalovirus-specific T cells for Clinical Applications using the Cytokine-capture System

Pappanaicken Kumaresan*¹, Mathew Figliola*¹, Judy S. Moyes, M. Helen Huls, Priti Tewari, Elizabeth J. Shpall, Richard Champlin, Laurence J.N. Cooper

¹Division of Pediatrics,U.T. MD Anderson Cancer Center, ²Stem Cell Transplantation and Cellular Therapy,U.T. MD Anderson Cancer Center

Journal (Developmental Biology)

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays

Cyrille Ramond³, Antonio Bandeira, Claire Berthault, Pablo Pereira, Ana Cumano, Odile Burlen-Defranoux

¹Research Center Growth and Signaling, INSERM U845,Institut Cochin, ²Unit for Biology of Lymphocyte Populations, Immunology Department, Institut Pasteur and CIMI, Unity of Treg Biology and Therapy,University of Pierre & Marie Curie, ³Unit for Lymphopoiesis, Immunology Department, INSERM U668,University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Institut Pasteur

Journal (Immunology and Infection)

Analyzing the Functions of Mast Cells In Vivo Using ‘Mast Cell Knock-in‘ Mice

Nicolas Gaudenzio, Riccardo Sibilano, Philipp Starkl, Mindy Tsai, Stephen J. Galli², Laurent L. Reber

¹Department of Pathology,Stanford University School of Medicine, ²Department of Microbiology & Immunology,Stanford University School of Medicine

Journal (Immunology and Infection)

Isolation and Transplantation of Different Aged Murine Thymic Grafts.

Y. Maurice Morillon II*¹, Fatima Manzoor*¹, Bo Wang, Roland Tisch

¹Department of Microbiology and Immunology,University of North Carolina at Chapel Hill

Journal (Biology)

Detection of miRNA Targets in High-throughput Using the 3’LIFE Assay

Justin M. Wolter², Kasuen Kotagama², Cody S. Babb, Marco Mangone²

¹School of Life Sciences,Arizona State University, ²Biodesign Institute,Arizona State University

Journal (Bioengineering)

Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding

Angela Rossi*¹, Antje Appelt-Menzel*¹, Szymon Kurdyn, Heike Walles², Florian Groeber

¹Department for Tissue Engineering and Regenerative Medicine,University Hospital Würzburg, ²Translational Center Würzburg, Regenerative Therapies in Oncology and Musculoskelettal Disease,Würzburg Branch of the Fraunhofer-Institute Interfacial Engineering and Biotechnology, IGB

Journal (Medicine)

Busulfan as a Myelosuppressive Agent for Generating Stable High-level Bone Marrow Chimerism in Mice

Kyle Peake, John Manning, Coral-Ann Lewis², Christine Barr, Fabio Rossi, Charles Krieger³

¹Department of Biomedical Physiology and Kinesiology,Simon Fraser University, ²The Biomedical Research Centre,University of British Columbia, ³Division of Neurology, Department of Medicine, Neuromuscular Disease Unit,VHHSC

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