Summary
Renal transplantation in mice is a technically challenging procedure that requires careful post-operative care and treatment for success.
Abstract
Renal orthotopic transplantation in mice is a technically challenging procedure. Although the first kidney transplants in mice were performed by Russell et al over 30 years ago (1) and refined by Zhang et al years later (2), few people in the world have mastered this procedure. In our laboratory we have successfully performed 1200 orthotopic kidney transplantations with > 90% survival rate. The key points for success include stringent control of reperfusion injury, bleeding and thrombosis, both during the procedure and post-transplantation, and use of 10-0 instead of 11-0 suture for anastomoses.
Post-operative care and treatment of the recipient is extremely important to transplant success and evaluation. All renal graft recipients receive antibiotics in the form of an injection of penicillin immediately post-transplant and sulfatrim in the drinking water continually. Overall animal health is evaluated daily and whole blood creatinine analyses are performed routinely with a portable I-STAT machine to assess graft function.
Protocol
Discussion
We have performed approximately 1200 cases of renal transplantation in mice with a technical success rate of >90%. Compared to heart transplantation, the success rate of kidney transplantation is usually low. However, our laboratory has maintained a high success rate (> 90%) of kidney transplantation. The key points for success are to reduce reperfusion injuries to both donor kidney and recipients, and minimize bleeding and thrombosis throughout the procedure and post-operatively. The following factors may be most …
Divulgazioni
The authors have nothing to disclose.
Acknowledgements
These studies were supported in part by funding by NIH R01 AI36532 to GAH. We would like to acknowledge the outstanding support in transplant pathology provided by Dr. Tibor Nadasdy, and the invaluable guidance and foresight provided by the late Dr. Charles G. Orosz in initiating this research effort.
Materials
| Material Name | Tipo | Company | Catalogue Number | Comment |
|---|---|---|---|---|
| i-STAT | Altro | HESKA | Cat 5101 | |
| i-STAT Cartridge | Altro | HESKA | CAT 5110-CREA | |
| Sulfatrim | Altro | Actavis | R02109 | |
| Isoflurane | Altro | NOVAPLUS | CA-0474 | |
| 10-0 Suture | Surgery | Sharpoint | AK0100 |
Riferimenti
- Russell, P. S., Chase, C. M., Colvin, R. B., Plate, J. M. D. Kidney transplants in mice. An analysis of the immune status of mice bearing long-term, H-2 incompatible transplants. JExpMed. 147, 1449-1468 (1978).
- Zhang, Z., Schlachta, C., Duff, J., Stiller, C., Grant, D., Zhong, R. Improved techniques for kidney transplantation in mice. Microsurgery. 16 (2), 103-109 (1995).
