Injection buffer (IB)
50 mM potassium glutamate
0.5 M MgCl2 (APPENDIX 2A)
Store up to 2 years at −20° C
G-buffer
2 mM Tris⋅Cl, pH 8.0 (APPENDIX 2A)
0.2 mM CaCl2 (APPENDIX 2A)
Add just before use:
0.2 mM ATP (see recipe for 100 mM)
0.5 mM 2-mercaptoethanol
Lim, J., Danuser, G. Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM). J. Vis. Exp. (30), e1325, doi:10.3791/1325 (2009).