小鼠结肠炎模型使用葡聚糖硫酸钠（DSS）
Summary
葡聚糖硫酸钠（DSS），在饮用水管理是一个既定的小鼠急性结肠炎的炎症损伤模型。该协议列出了DSS的治疗方法和组织编制。
Abstract
结肠炎可发生病毒或细菌感染，缺血性侮辱，或自身免疫性疾病，最显着的溃疡性结肠炎和克罗恩病的结肠变种 – 克罗恩结肠炎。急性结肠炎患者可出现腹痛，腹胀，吸收不良，腹泻，便血和大便粘液。我们开始认识到环境，遗传学，和上皮屏障功能障碍在炎性肠病结肠炎动物模型已在推进我们对本病的认识至关重要之间复杂的相互作用。一种流行的模式涉及与低分子右旋糖酐硫酸钠（DSS）的补充对小鼠的饮用水，从而导致在结肠上皮损伤和一个强大的炎症反应，持续数天<sup> 1</sup>这种方法的变化可用于模型急性损伤，急性损伤所修复，和决策支持系统的建模与恢复的慢性炎症性疾病反复穿插周期<sup> 2</sup>。 3％DSS的饮用水中单一的为期四天的治疗后，小鼠急性结肠炎的迹象，包括体重减轻，便血，腹泻。小鼠在疗程结束时被实施安乐死，并在尸检解剖冒号处理，可'瑞士推出“<sup> 3</sup>，让整个结肠的微观分析，或注入福尔马林“香肠”，让宏观的分析。组织，然后嵌入石蜡，切片，染色病理审查。
Protocol
第1部分：损伤修复结肠炎模型基线体重应获得每个鼠标之前开始直接资助的治疗。 设为3％（W / V）葡聚糖在水硫酸钠盐溶液，并用0.45μm的醋酸纤维素过滤器的过滤器。 四天的3％DSS解决方案，更换鼠笼的饮用水。这些老鼠不应该有任何其他来源的水（即排除放置在自动浇水系统的提示）。 第四天，一个额外的三天更换水DSS解决方案，允许一些结肠上皮复苏。 4天小鼠?…
Discussion
这个协议可以进行修改，以急性损伤，损伤修复，或慢性结肠损伤过程模型。
急性损伤的修改：
决策支持系统治疗4-6天，立即牺牲即兴
损伤修复的修改：
伤4-6天3-4天，和牺牲的恢复期间（如在上述协议中所述）的DSS的治疗。
慢性结肠炎的修改：
小鼠被放置在三个为期五天的3?…
Divulgazioni
The authors have nothing to disclose.
Acknowledgements
由美国国立卫生研究院（1 K08 DK080221 – 01）和范德比尔特机构发展基金提供的资金。
Materials
| Material Name | Tipo | Company | Catalogue Number | Comment |
|---|---|---|---|---|
| Dextran Sulfate Sodium Salt | USB Corp | 9011-18-1 | mol wt 40,000-50,000 | |
| 10% Buffered Formalin Phosphate | Fisher Scientific | SF100-4 | ||
| Isoflurane, USP | Phoenix Pharmaceuticals | NDC 57319-474-06 | ||
| TISSUE PATH Macrosette Cassettes | Fisher Healthcare | 15182706 | ||
| BD 10 ml Syringe | B-D | 309604 | ||
| Ethyl Alcohol | Pharmco-Aaper | E190 | Dilute to 70% with distilled water | |
| 20G Straight Feeding Needle | VWR | 20068-612 | ||
| 27G1/2 PrecisionGlide Needle | B-D | 305109 | ||
| Dissecting Scissors Sharp/Blunt Tip | VWR | 82027-588 | ||
| Waugh Forceps | VWR | 82027-428 | ||
| Non-absorbable Suture | LOOK Surgical Specialties | SP116 | ||
| Whatman Blotting Paper | VWR | 28298-020 | Cut as needed | |
| Nalgene Surfactant-Free Cellulose Acetate (SFCA) Filter | Cole-Parmer | EW-06731-2 | ||
| Carbon fiber composites digital caliper | Fisher Scientific | 15-007-958 |
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Tags
Murine Colitis ModelingDextran Sulfate Sodium (DSS)ColitisUlcerative ColitisCrohn’s DiseaseCrohn’s ColitisAbdominal PainDistentionMalabsorptionDiarrheaHematocheziaMucus In StoolAmbienteGeneticaEpithelial Barrier DysfunctionInflammatory Bowel DiseaseAnimal Models Of ColitisLow-molecular Weight Dextran Sodium Sulfate (DSS)Epithelial DamageInflammatory ResponseAcute InjuryChronic Inflammatory Diseases3% DSS In Drinking WaterWeight LossBloody StoolsDiarrheaEuthanizedNecropsy Dissected ColonsMicroscopic AnalysisMacroscopic Analysis
Citazione di questo articolo
Whittem, C. G., Williams, A. D., Williams, C. S. Murine Colitis Modeling using Dextran Sulfate Sodium (DSS). J. Vis. Exp. (35), e1652, doi:10.3791/1652 (2010).