We demonstrate the utility of multicolor flow cytometry for detailed phenotypic and functional characterization of total as well as memory subsets of CD4+ and CD8+ T cells in rhesus macaques, the ideal model for HIV/AIDS vaccine studies.
Dr. Pramod Nehete and Mrs. Bharti Nehete for macaque blood and PBMC samples; This work was partially supported by funds from NIH grant AI 46969, and all the cell culture media were produced by the Central Media Laboratory, which is funded by NIH grant CA 16672.
Solution and Buffers
Activation agents
Cytokine secretion inhibitors
Antibodies