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¹³C₄-天冬氨酸和 ¹³C₅-谷氨酰胺稳定同位素示踪、LC-MS 分析和 SDH 活性测量

Published: May 19, 2023

doi:

Tenzin Tseyang*¹, Jonathan Valeros*¹, Paula Vo*¹, Jessica B. Spinelli²

¹Program in Molecular Medicine,University of Massachusetts Chan Medical School, ²Cancer Center,University of Massachusetts Chan Medical School

Tags

13C4-aspartate 13C5-glutamine Stable Isotope Tracing LC-MS Analysis SDH Activity Measurement 143B Human Osteosarcoma Cells Cell Culture Metabolite Extraction Vacuum Concentration Liquid Chromatography Mass Spectrometry

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DOI

Citazione di questo articolo

¹³C₄-Aspartate and ¹³C₅-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement. J. Vis. Exp. (Pending Publication), e200017, doi:10.3791/200017 (2023).

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