Generation of Rat Intestinal 2D Monolayers from 3D Organoids - JoVE

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Generation of Rat Intestinal 2D Monolayers from 3D Organoids (Video) | JoVE

Published: June 23, 2023

doi:

Eleanor Zagoren*¹, Anderson K. Santos*^1,2, Nadia A. Ameen⁴, Kaelyn Sumigray^5,6

¹Department of Genetics,Yale School of Medicine, ²Department of Pediatrics,Yale School of Medicine, ³Department of Pediatrics/Gastroenterology and Hepatology,Yale School of Medicine, ⁴Department of Cellular and Molecular Physiology,Yale School of Medicine, ⁵Yale Stem Cell Center,Yale School of Medicine, ⁶Yale Cancer Center,Yale School of Medicine

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Keywords: Rat Intestinal 2D Monolayers 3D Organoids EME Coating Collagen Coating Organoid Disruption Trypsin Treatment RIOM2D Y27632 2D Monolayers 3D Structures

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Citazione di questo articolo

Generation of Rat Intestinal 2D Monolayers from 3D Organoids. J. Vis. Exp. (Pending Publication), e200274, doi:10.3791/200274 (2023).

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