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Rilevamento di proteine in cellule di adenocarcinoma dello stomaco trasfettate TMEM200A siRNA mediante Western Blotting

Published: September 15, 2023

doi:

Yaowen Zhang, Shanshan Kuang, Hancheng Qin, Na Zhao, Yixin Yang, Jisheng Xie

¹Department of Histology and Embryology,Youjiang Medical University for Nationalities, ²Department of Pathophysiology,Youjiang Medical University for Nationalities

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Keywords: TMEM200A SiRNA Stomach Adenocarcinoma Western Blotting Protein Detection Cell Culture PBS Radioimmune Precipitation Assay BCA Protein Assay SDS-PAGE PVDF Membrane TBST Blocking Buffer Primary Antibody Secondary Antibody ECL Substrate Epithelial Mesenchymal Transition Phosphoinositide 3-kinase Signaling Pathway

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DOI

Citazione di questo articolo

Protein Detection in TMEM200A siRNA Transfected Stomach Adenocarcinoma Cells Using Western Blotting. J. Vis. Exp. (Pending Publication), e200744, doi:10.3791/200744 (2023).

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