Isolation and Cryopreservation of Human Peripheral Blood Mononuclear Cells for Bioenergetic Studies

doi:10.3791/200888

JoVE Journal > Immunology and Infection

Immunologia e infezioni

Isolation and Cryopreservation of Human Peripheral Blood Mononuclear Cells for Bioenergetic Studies

Published: October 20, 2023

doi:

10.3791/200888

Fabian Dieter, Jendrik Grube, Tim Birkenhauer, Alice Quentin, Gunter P. Eckert

¹Biomedical Research Center, Institute of Nutritional Sciences,Justus-Liebig-University of Giessen

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Isolation Cryopreservation Peripheral Blood Mononuclear Cells PBMC Bioenergetic Studies DPBS Lymphocyte Isolation Medium Centrifugation Plasma Layer Resuspension Fetal Bovine Serum DMSO Cryotubes Freezing Liquid Nitrogen

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Isolation and Cryopreservation of Human Peripheral Blood Mononuclear Cells for Bioenergetic Studies. J. Vis. Exp. (Pending Publication), e200888, doi:10.3791/200888 (2023).

Isolation and Cryopreservation of Human Peripheral Blood Mononuclear Cells for Bioenergetic Studies

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