Preparation of Human Intestinal Organoid Monolayers for Live Fluorescence Imaging (Video) | JoVE

Published: January 19, 2024

doi:

10.3791/201231

J. Thomas Gebert, Francesca J. Scribano, Kristen A. Engevik, Joseph M. Hyser

¹Alkek Center for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology,Baylor College of Medicine

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Keywords: Intestinal Organoid Monolayer Live Fluorescence Imaging Collagen IV EDTA PBS Enzymatic Dissociation CMGF WRNE Medium Cell Culture Differentiation Medium

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Preparation of Human Intestinal Organoid Monolayers for Live Fluorescence Imaging. J. Vis. Exp. (Pending Publication), e201231, doi:10.3791/201231 (2024).

Preparation of Human Intestinal Organoid Monolayers for Live Fluorescence Imaging (Video) | JoVE

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