Preparation of Drosophila Heart: A Technique to Study Heart Physiology in the Adult Fly
Published: April 30, 2023
Abstract
Source: Vogler, G., Ocorr, K. Visualizing the Beating Heart in Drosophila. J. Vis. Exp. (2009).
This video describes the fly's adult heart, or otherwise called the Drosophila dorsal vessel. The featured protocol demonstrates a dissection procedure for live heart preparations.
Protocol
This protocol is an excerpt from Vogler and Ocorr, Visualizing the Beating Heart in Drosophila, J. Vis. Exp. (2009). 1. Before you start Freshly prepare artificial hemolymph (AH) solution containing 108mM Na+, 5mM K+, 2mM Ca2+, 8mM MgCl2, 1mM NaH2PO4, 4mM NaHCO3, 10mM sucrose, 5mM trehalose, 5mM HEPES (pH 7.1, all reagents from Sigma Chemicals). The sucrose and trehalose sho…
Materials
| Micro Dissecting Spring Scissors (curved) | Roboz Surgical Instruments Co. | RS-5611 | Good for gross cuts (Step 2) |
| Micro Dissecting Spring Scissors (straight) | Roboz Surgical Instruments Co. | RS-5620 | |
| Dumont #55 forceps | Fine Science Tools | 11295-51 | |
| Dumont #5 forceps | Fine Science Tools | 11295-10 | |
| Glass Capillaries, fine | Science Products | GB100T8P | |
| Pipette Puller | Sutter Equipment | P-97 | Both horizontal and vertical pipette pullers will work |
| Plastic tubing | Tygon | 1/16" inner diameter | for 100 µl capillaries |
| Histoacryl® tissue adhesive | B. Braun Medical |
Citazione di questo articolo
Preparation of Drosophila Heart: A Technique to Study Heart Physiology in the Adult Fly. J. Vis. Exp. (Pending Publication), e20151, doi: (2023).