Preparing HASM Cells for cAMP Detection Using Inverted Fluorescence Microscopy

doi:10.3791/201671

JoVE Journal > Biochemistry

Biochimica

Preparing HASM Cells for cAMP Detection Using Inverted Fluorescence Microscopy

Published: March 22, 2024

doi:

10.3791/201671

Isabella Cattani-Cavalieri, Jordyn Margolis, Camelia Anicolaesei, Francisco J. Nuñez, Rennolds S. Ostrom

¹Department of Biomedical and Pharmaceutical Sciences,Chapman University School of Pharmacy

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CAMP Biosensor CADDis Epac2 Fluorescent Protein CAMP Dynamics Real-time Measurement HEK-293 Cells HASM Cells 96-well Plate Fluorescent Microscopy High-throughput Screening CAMP Signaling

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Preparing HASM Cells for cAMP Detection Using Inverted Fluorescence Microscopy. J. Vis. Exp. (Pending Publication), e201671, doi:10.3791/201671 (2024).

Preparing HASM Cells for cAMP Detection Using Inverted Fluorescence Microscopy

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