DNA Extraction from Tail Clip: A Method Used in Zebrafish Genotyping

Abstract

Source: Xing L., et al. Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis. J. Vis. Exp. (2014).

This article describes DNA extraction from zebrafish tail clip. The protocol demonstrates a rapid and efficient method to analyze zebrafish genotypes.

Protocol

1. DNA Preparation Prepare DNA lysis buffer: 50 mM KCl, 10 mM Tris-HCl pH 8.3, 0.3% Tween 20, 0.3% NP40. Add fresh Proteinase K to a final concentration of 1 mg/ml on the day of use. Tissue collection: For adult fish fin clip: Anesthetize fish: Place the fish in 0.004% MS-222 (tricaine) solution. Wait until gill movement slows. Put fish on a stack of 5-10 Kimwipes and cut a small piece of the tail fin, about 2-3 mm, with a sterile razor blade. Quickl…

Materials

100 Reaction LightScanner Master Mix	BioFire	HRLS-ASY-0002	www.biofiredx.com
Tricaine
Paraformaldehyde