Orthotopic Injection: Implanting Tissue Specific Cancer Cells into an Adult Mouse
Published: April 30, 2023
Abstract
Source: Katsuta, E., et al. Generating a Murine Orthotopic Metastatic Breast Cancer Model and Performing Murine Radical Mastectomy. J. Vis. Exp. (2018).
The video demonstrates the breast cancer orthotopic inoculation technique in a mouse model. This technique produces a high tumorigenesis rate with less variability in tumor size and shape compared to subcutaneous or non-surgical injection.
Protocol
1. Preparation of Cells (for 10 Mice) NOTE: The cells should be inoculated within 1 h after being detached from the dish to avoid decreased cell viability. Specifically, the cell suspension should be mixed into the gelatinous protein mixture within 15 min after detaching the cells from the dish to maintain their viability. Culture 4T1-luc2 cells, a mouse mammary adenocarcinoma cell line expressing luciferase, in RPMI 1640 media with 10% FBS in a humidified inc…
Divulgazioni
The authors have nothing to disclose.
Materials
| Micro Dissection Scissors | Roboz | RS-5983 | For cancer cell innoculation and mastectomy |
| 5-0 silk sutures | Look | 774B F | |
| Roswell Park Memorial Insitute 1640 | Gibco | 11875093 | For cell culture |
| Fetal Bovine Serum | Gibco | 10437028 | For cell culture |
| Trypsin-EDTA (0.25%) | Gibco | 25200056 | For cell culture |
| Dry sterilant (Germinator 500) | Braintree Scientific | GER 5287-120V | For cancer cell innoculation and mastectomy |
| Needle Holder | Roboz | RS-7830 | For cancer cell innoculation and mastectomy |
| Adson Forceps | Roboz | RS-5233 | For cancer cell innoculation and mastectomy |
| Clipper | Wahl | 9908-717 | For cancer cell innoculation and mastectomy |
| Matrigel | Corning | 354234 | For cancer cell innoculation |
Citazione di questo articolo
Orthotopic Injection: Implanting Tissue Specific Cancer Cells into an Adult Mouse. J. Vis. Exp. (Pending Publication), e20212, doi: (2023).