Ortopisk injektion: Implantering vævsspecifikke kræftceller i en voksen mus
Published: April 30, 2023
Abstract
Kilde: Katsuta, E., et al. Generering af en Murine Orthotopic Metastatic Breast Cancer Model og Udfører Murine Radical Mastectomy. J. Vis. Exp. (2018).
Videoen viser brystkræft orthotopic podning teknik i en mus model. Denne teknik producerer en høj tumorigenese sats med mindre variation i tumor størrelse og form i forhold til subkutane eller ikke-kirurgisk injektion.
Protocol
1. Forberedelse af celler (til 10 mus) BEMÆRK: Cellerne skal vaccineres inden for 1 time efter at være blevet løsrevet fra skålen for at undgå nedsat celle levedygtighed. Specifikt bør celleaffjedringen blandes i den gelatineholdige proteinblanding inden for 15 minutter efter at have løsnet cellerne fra skålen for at opretholde deres levedygtighed. Kultur 4T1-luc2 celler, en mus mammary adenocarcinoma celle linje udtrykke luciferase, i RPMI 1640 medier med 1…
Divulgazioni
The authors have nothing to disclose.
Materials
| Micro Dissection Scissors | Roboz | RS-5983 | For cancer cell innoculation and mastectomy |
| 5-0 silk sutures | Look | 774B F | |
| Roswell Park Memorial Insitute 1640 | Gibco | 11875093 | For cell culture |
| Fetal Bovine Serum | Gibco | 10437028 | For cell culture |
| Trypsin-EDTA (0.25%) | Gibco | 25200056 | For cell culture |
| Dry sterilant (Germinator 500) | Braintree Scientific | GER 5287-120V | For cancer cell innoculation and mastectomy |
| Needle Holder | Roboz | RS-7830 | For cancer cell innoculation and mastectomy |
| Adson Forceps | Roboz | RS-5233 | For cancer cell innoculation and mastectomy |
| Clipper | Wahl | 9908-717 | For cancer cell innoculation and mastectomy |
| Matrigel | Corning | 354234 | For cancer cell innoculation |
Citazione di questo articolo
Orthotopic Injection: Implanting Tissue Specific Cancer Cells into an Adult Mouse. J. Vis. Exp. (Pending Publication), e20212, doi: (2023).