Vitrification-based Cryopreservation: An Ultra-rapid Cooling Technique to Preserve Biological Specimens at Very Low Temperatures for Long-term Storage
Published: April 30, 2023
Abstract
Source: Lu, J. H. et al. Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines. J. Vis. Exp. (2019)
In this video, we demonstrate an ultra-rapid cryo vitrification method for preserving biological samples. The method entails the use of a combination of cryoprotectants to protect the biological tissue from freezing damage by preventing intracellular ice crystal formation.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Tissue Cryopreservation NOTE: This part primarily references the methods for the Live Tissue Kit Cryo Kit. The main kits and equipment are listed in the Table of Materials. Euthanize the mice with an IRB-approved method when the tumor is greater than 10 …
Divulgazioni
The authors have nothing to disclose.
Materials
| Live Tissue Kit Cryo Kit | Celliver Biotechnology, Shanghai, China | LT2601 | |
| Tissue-processed molds and auxiliary blades | Celliver Biotechnology, Shanghai, China | LT2603 | |
| DPBS | Basalmedia Technology, Shanghai, China | L40601 |
Tags
Citazione di questo articolo
Vitrification-based Cryopreservation: An Ultra-rapid Cooling Technique to Preserve Biological Specimens at Very Low Temperatures for Long-term Storage. J. Vis. Exp. (Pending Publication), e20413, doi: (2023).