This video describes the preparation of a decellularized rat kidney. The obtained acellular vascular scaffold can serve as a promising platform for tissue engineering of organ grafts.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Kidney Harvesting and Decellularization Harvest the left kidney with the abdominal aorta and inferior vena cava. Ligate the ureter, thoracic aorta, superior vena cava, and branches of the abdominal aorta. Keep the organ hydrated in Dulbecco's PBS (DPBS) in a 10 cm Petri dish. Cannulate …
Divulgazioni
The authors have nothing to disclose.
Materials
Polyethelyne 50 tubing, catheter tubing 100 ft
Braintree Scientific
.023" × .038”
3-0 silk spool vascular access/ ligation in rat
Braintree Scientific
SUT-S 110
Fine scissors to cut fascia/ connective tissue
Fine Science Tools
14058-09
25 gauge inch guide needle(for vascular catheters)
Preparation of Decellularized Rat Kidney: A Procedure to Generate Acellular Vascularized Whole-organ Scaffold from a Harvested Rat Kidney. J. Vis. Exp. (Pending Publication), e20552, doi: (2023).