Fluorescence Molecular Tomography: An Imaging Technique for In Vivo Imaging of Fluorescent Protein-tagged Glioblastoma Xenografts in Mouse Model

Abstract

Source: Benitez, J. A. et al., Fluorescence Molecular Tomography for In Vivo Imaging of Glioblastoma Xenografts. J. Vis. Exp. (2018)

In this video, we describe fluorescence molecular tomography or FMT for in vivo imaging of fluorescent protein-tagged glioblastoma xenografts in mouse models. The technique is useful for elucidating the effects of anti-tumor therapeutics at the molecular level.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1.FMT Imaging

NOTE: According to the experimental aim, the iRFP-tagged glioblastoma cells can be monitored in vivo before, during, or after treatment. For imaging purpose, anesthetize animals using an isoflurane induction chamber, maintain in an imaging cassette during the scanning, and image in the docking station of the FMT imager.

1. Remove the animal from the cage and place in the isoflurane induction chamber. Close the cham....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Fluorescence Tomography System	PerkinElmer	FMT 2500 XL
TrueQuant Imaging Software	Perkin Elmer	7005319
Athymic nude mice	Charles River Laboratories	Strain Code 490 (Homozygous)	Prior approval by the Institutional Animal Care Program and by the Institutional Biosafety Committee required.