This video demonstrates the isolation of intact vessels from a mouse's brain that constitute the blood-brain barrier (BBB) along with neurons and glial cells. The isolated vessels can be used as an in vitro model to study their role in maintaining brain homeostasis.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Dissection NOTE: Sterile conditions are not required unless vessels are used for cell culture purposes. Prepare isolation vessel solutions: B1, add 1.5 mL of HEPES 1M to 150 mL of HBSS; B2, add 3.6 g of Dextran to 20 mL of B1; B3, add 1 g of BSA to 100 mL of B1. …