Modeling Photothrombotic Stroke in Mouse Model: A Laser Illumination Technique Through Mouse Skull Following Photosensitive Dye Administration to Induce Photothrombosis

Abstract

Source: Llovera, G. et al. Modeling Stroke in Mice: Focal Cortical Lesions by Photothrombosis. J. Vis. Exp. (2021)

This video demonstrates the procedure to generate photothrombotic stroke in a mouse model by laser illumination of an intact skull following administration of photosensitive dye.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. Preparation of the animal

1. Inject analgesia 30 min before surgery (4 mg/kg Carprofen and 0.1 mg/kg Buprenorphine).
2. Record the mouse body weight to adjust the dose of Rose Bengal to be injected (10 µL/g i.e., 100 µg/g).
3. Place the mouse in the induction chamber with an isoflurane flow rate of 4% to anesthetize it until the spontaneous movement of the body and vibrissae stops.
4. Transfer the mouse into the stereotactic frame and plac....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
561 nm wavelenght laser	Solna	Cobolt HS-03
C57Bl/6J mice	Charles River	664
Collimeter	Thorlabs	F240APC-A
Cotons	NOBA Verbondmitel Danz	974116
Ethanol 70%	CLN Chemikalien Laborbedorf	521005
Fine Scissors	FST	15000-00
Forceps	FST	11616-15
Heating blanket	FHC DC Temperature Controller	40-90-8D
Isoflurane	Abbot	B506
Protective glasses	Laser 2000	NIR-ZS2-38
Rose Bengal	Sigma Aldrich	198250-5G
Saline solution	Braun	131321
Stereotactic frame	Stoelting	51500U
Anesthesia system for isoflurane	Drager
Phosphate Buffered Saline PH: 7.4	Apotheke Innestadt Uni Munchen	P32799