Nictitating Membrane Removal: A Surgical Procedure to Remove Nictitating Membrane from Rabbit Eye

Published: April 30, 2023

Abstract

Source: Honkanen, R. A., et al. Establishment of a Severe Dry Eye Model Using Complete Dacryoadenectomy in Rabbits. J. Vis. Exp. (2020).

In this video, we demonstrate a surgical procedure to remove nictitating membrane from rabbit’s eye.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Removal of the nictitating membrane NOTE: For simplicity, the technique for the right eye is described below. Complete this procedure on the left eye in an identical manner. Remove the nictitating membrane bilaterally during the acclimation period (usually the first week). Place rabbit in an …

Divulgazioni

The authors have nothing to disclose.

Materials

acepromazine, Aceproinj Henry Schein Animal Health, Dublin, OH NDC11695-0079-8 0.1ml/kg subcutaneously injection for rabbit sedation
animal restraining bag Henry Schein Animal Health, Dublin, OH Jorvet J0170 Use appropriately sized bag.
cautery unit, high-temperature, battery-powered Medline Industries Inc, Northfield, IL REF ESCT001 Keep on hand in case of bleeding, protocol 2.7
forceps, 0.3 Bausch and Lomb (Storz), Bridgewater, NJ ET6319 For removal of nictating membrane, protocol 2.5
lid speculum, wire Bausch and Lomb (Storz), Bridgewater, NJ Barraquer SUH01 For removal of nictating membrane, protocol 2.4
lidocaine 2% with epinephrine 1:100,000; 50:50 mixture Hospira Inc, Lake Forest IL NDC 0409-3182-02 Pre-treat before removal of nictating membranes, protocol 2.4
lidocaine, preservative-free Sigma-Aldrich, St. Louis, MO L5647 1% in PBS for anesthesia agent, for application to eye, protocol 2.4
micropipette Eppendorf Research Plus 100 uL For application of preservative-free lidocaine to eye, protocol 2.4
micropipette tips World Wide Medical Products 41071052 For application of preservative-free lidocaine to eye, protocol 2.4
needle, 26-gauge BD, Franklin Lakes, NJ REF 305115 For injection of lidocaine/epinephrine, protocol 2.3 & 2.5
rabbit, New Zealand White Charles River Labs, Waltham, MA (NZW) 2-3 kg Research animals
scissors, Westcott tenotomy McKesson Medical-Surgical, San Francisco, CA Miltex 18-1480 For removal of nictating membrane, protocol 2.7
syringe, 1 cc BD, Franklin Lakes, NJ ref 309659 For injection of lidocaine/epinephrine, protocol 2.3 & 2.5

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Citazione di questo articolo
Nictitating Membrane Removal: A Surgical Procedure to Remove Nictitating Membrane from Rabbit Eye. J. Vis. Exp. (Pending Publication), e20845, doi: (2023).

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