TTC Staining of Rat Brain Tissue Slices: A Staining Procedure to Differentiate Viable and Necrotic Tissue in Tissue Slices After Ischemia-Reperfusion Injury

Published: April 30, 2023

Abstract

Source: Liepinsh, E., et al. Rodent Heart and Brain Tissue Preparation for Digital Macro Photography after Ischemia-reperfusion. J. Vis. Exp. (2022)

This video describes the 2,3,5-triphenyl-2H-tetrazolium chloride staining procedure to visualize rat brain tissue slices and differentiate viable and damaged tissue following middle cerebral artery occlusion and reperfusion.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Brain staining and slicing After the middle cerebral artery occlusion experiment, remove the brain, including the brainstem, from the skull, and wash it in ice-cold PBS. Choose the correct size of the brain stainless-steel matrix (see the Table of Materials) depending on the weight of the anim…

Representative Results

Figure 1: Matrices for the rat heart and brain slicing. (A) Rat heart, (B) rat brain.

Divulgazioni

The authors have nothing to disclose.

Materials

2,3,5-Triphenyltetrazolium chloride (TTC) Sigma-Aldrich 298-96-4
Adult Rat Brain Slicer Matrix Zivic Instruments BSRAS001-1
Single Edge Razor Blades Zivic Instruments BLADE012.1
Sony Alpha a6000 Mirrorless Digital Camera Sony ILCE6000 Can be repalaced by any up-to-date digiatal camera
Surgical blade Heinz Herenz Hamburg Germany BS2982
Weigh tray, 70 mL Sarsted 71,99,23,212
Thermo-Shaker BioSan PST-60HL-4
Toothed tissue forceps Agnthos 11021-12
Sodium chloride Fisher bioreagents BP358-10
Cover Glass Forceps, Angled Fine Science Tools 11073-10

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Citazione di questo articolo
TTC Staining of Rat Brain Tissue Slices: A Staining Procedure to Differentiate Viable and Necrotic Tissue in Tissue Slices After Ischemia-Reperfusion Injury. J. Vis. Exp. (Pending Publication), e21046, doi: (2023).

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