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鼠类全胚胎培养方法，使用的肩型瓶培养系统
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Biologia
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JoVE Journal Biologia
The Method of Rodent Whole Embryo Culture using the Rotator-type Bottle Culture System
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鼠类全胚胎培养方法，使用的肩型瓶培养系统

DOI:
10.3791/2170-v

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10:04 min

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August 28, 2010

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1Division of Developmental Neuroscience, United Centers for Advanced Research and Translational Medicine (ART),Tohoku University Graduate School of Medicine, 2The Core Research for Evolutional Science and Technology (CREST),Japan Science and Technology Corporation (JST)

Capitoli

  • 00:00The Method of Rodent Whole Embryo Culture Using the Rotator-type Culture System
  • 00:07Introduction
  • 02:06Setting up Whole Embryo Culture System
  • 02:52Preparation of Culture Medium
  • 03:53Removal of Embryos from the Uterus
  • 04:52Dissection of Rat Embryos
  • 07:27Results and Discussion

Summary

Traduzione automatica

Traduzione automatica

全胚胎培养技术，使我们文化的大鼠和小鼠胚胎<em>体外</em>在midgestation阶段对应有限的时期条件。在这个视频协议，我们将演示后，用肩瓶型文化体系E12.5大鼠全胚胎培养我们的标准程序。

Tags

Rodent Whole Embryo CultureWEC TechniqueRotator-type Bottle Culture SystemBiologia dello sviluppoMouse EmbryosRat EmbryosEx Vivo ConditionEmbryonic DayPlacenta FunctionManipulation Under MicroscopeDynamic Developmental ProcessesPostimplanted Mammalian EmbryosLong-term CultureRat WEC After E12.5Refined ModelApplications In Mammalian Developmental Biology

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