Oral Intubation of Adult Zebrafish to Administer Experimental Compounds

Abstract

Source: Ji, J., Thwaite, R. et al. Oral Intubation of Adult Zebrafish: A Model for Evaluating Intestinal Uptake of Bioactive Compounds. J. Vis. Exp. (2018).

This video demonstrates a technique for orally intubating adult zebrafish to administer experimental compounds into the esophagus and monitor their intestinal uptake. This method offers a valuable model for studying the dynamics of oral prophylactics or other compounds within the intestine.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board

1. Solutions Required

1. Prepare 150 mg/L (for anesthesia) or 300 mg/L (for euthanasia) of ethyl 3-aminobenzoate methanesulfonate (MS-222) solution with water from the aquarium where the zebrafish are maintained. Fill a small tank with 1 L of anesthetic solution and keep it aerated.
2. Fill another small tank with 1 L of aquarium water without MS-222 for fish recovery and keep it aerated.
3. Make 50 mL of 1x PBS from a 10x sterile stock solution.
4. For....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
Silicone tube	Dow Corning	508-001	0.30 mm inner diameter and 0.64 mm outer diameter
Luer lock needle	Hamilton	7750-22	31 G, Kel-F Hub
Luer lock syringe	Hamilton	81020/01	100 μL, Kel-F Hub
Filtered pipette tip	Nerbe Plus	07-613-8300	10 μL
MS-222	Sigma Aldrich	E10521	powder
10x PBS	Sigma Aldrich	P5493
Filter paper	Filter-Lab	RM14034252
Atto-488 NHS ester	Sigma-Aldrich	41698
DMEM	Gibco	31966	Dulbecco's modified eagle medium
Maxwell RSC simplyRNA Tissue Kit	Promega	AS1340