An In Vivo PET Imaging Technique to Detect Tumors in a Murine Model Using Radiolabeled Antibodies

Abstract

Source: Zeglis, B. M., et al. The Bioconjugation and Radiosynthesis of ⁸⁹Zr-DFO-labeled Antibodies. J. Vis. Exp. (2015).

This video demonstrates a technique involving a radioimmunoconjugate for the in vivo imaging of a tumor in a mouse model. Upon injection of the radioimmunoconjugate, the antibodies of the conjugate bind to a specific membrane antigen on the cancer cells. The radiotracer of the conjugate emits positrons that interact with neighboring electrons to produce energy, which is detected by a positron emission tomography (PET) scanner to create an image of the tumor.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.

1. In Vivo PET Imaging with ⁸⁹Zr-DFO-J591

CAUTION: This step of the protocol involves the handling and manipulation of radioactivity. Before performing these steps, researchers should consult with their home institution's Radiation Safety Department. All possible steps should be taken to minimize exposure to ionizing radiation.

1. In male athymic nude mice, subcutaneously implant 5 x 10⁶ lymph node....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
p-SCN-Bn-DFO	Macrocyclics	B-705	Store at -80 °C
[89Zr]Zr-oxalate	Various, including Perkin-Elmer	-	Caution: Radioactive material