This video demonstrates a technique for the continuous infusion of amyloid-beta (Aβ) peptide-specific antibodies into the hippocampus of a rat. The study employs implanting a bilateral cannula system for a controlled infusion of Aβ peptide-specific antibodies coupled with the infusion of soluble Aβ oligomers (Aβo) into the rat hippocampus to study the interactions between the oligomers and the antibodies.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Catheter Preparation before Stereotaxic Surgery Cut PE50 catheters (6 cm in length) that will be used to connect the cannula with osmotic pumps (see Figure 1). Fill PE50 catheters with artificial cerebrospinal fluid (aCSF) and seal both ends of the catheters. Keep the catheter at 4 °C until used.</…
Representative Results
Figure 1. Photo of the Animal with Bilateral Cannula During Aβo Infusion. A solution of Aβo (0.2 µg/µl; 1 µl) was injected in awake and freely moving rats (once a day for 6 days) using PE50 catheters connected to an internal cannula inserted into a guide cannula. Treatment with control (Ctl) IgG1 or 6E10 antibody was infused directly at the site of Aβ…
Divulgazioni
The authors have nothing to disclose.
Materials
Artificial Cerebrospinal Fluid (aCSF)
Harvard Apparatus
59-7316
PE50 Catheter thin wall
Plastics one
C232CT
Ketamine Hydrochloride (100 mg/mL)
Bioniche
1989529
Xylaxine Hydrochloride (100 mg/mL)
Bimeda
8XYL004C
Meloxicam (5 mg/mL)
Norbrook
215670I01
Solution of chlorhexidine gluconate 2% and isopropyl alcohol 2%
Controlled Infusion of Aβ Peptide-Specific Antibodies and Aβ Oligomers into the Rat Hippocampus. J. Vis. Exp. (Pending Publication), e22127, doi: (2024).