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一个孤立的视网膜制备,记录从基因标记的视网膜神经节细胞的光反应
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
An Isolated Retinal Preparation to Record Light Response from Genetically Labeled Retinal Ganglion Cells
DOI:

13:02 min

January 26, 2011

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Capitoli

  • 00:05Titolo
  • 01:01Introduction
  • 01:33Preparation of Solutions
  • 02:29Isolation of Retina from Mouse Eye
  • 04:08Treatment of Retina with Enzyme MIxture and Mounting in Recording Chamber
  • 06:10Localization of EGFP Expressing Ganglion Cells and Light Stimulation
  • 09:51Analysis of Anatomy of Recorded Ganglion Cells
  • 11:21Response of Retinal Ganglion Cell to Light Stimulation and Correlation with Morphology
  • 12:45Conclusion

Summary

Traduzione automatica

本文提供了一个描述如何从孤立在小鼠视网膜准备的解剖和记录。特别是,我们介绍了如何从一个荧光标记的神经节细胞的人口记录对光反应和随后查明并分析其形态。

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