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测定在异种细胞表面的化学感应受体的表达
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JoVE Journal Neuroscienze
Assaying Surface Expression of Chemosensory Receptors in Heterologous Cells
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测定在异种细胞表面的化学感应受体的表达

DOI:
10.3791/2405-v

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04:55 min

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February 23, 2011

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, ,
1Department of Molecular Genetics and Molecular Biology ,Duke University, 2Department of Chemistry,Duke University

Capitoli

  • 00:05Titolo
  • 01:25Transfection of HEK293T Cells and Plating for Surface Expression Assay
  • 02:47Cell Surface Staining of Receptors
  • 04:12Cell Surface Staining of the Odorant Receptor Olfr62
  • 04:27Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里，我们展示了一个协议，进行活细胞染色，可用于检测HEK293T细胞表面上的气味受体方便。此外，它也可能被用来检测其他化学感受受体或GPCRs的表面表达。

Tags

AssayingSurface ExpressionChemosensory ReceptorsHeterologous CellsOlfactionG Protein Coupled Receptors (GPCRs)Odorant MoleculesIntracellular SignalG Protein GαolfCombinatorial RuleBiochemical StudiesLigand Binding StudiesHeterologous CellsReceptor Transporting Protein (RTP)Olfactory Sensory NeuronsChaperonesTransfectionBiochemical AssaysCell LineLive Cell Staining

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