自体血液的注入模型小鼠自发性脑出血
Summary
自体血注射在本议定书中所描述的小鼠脑出血模型，采用双色注塑技术，以最大限度地减少了针轨道，在泵送系统中没有抗凝血剂的血液回流的风险，并消除了所有死在空间和系统扩展的油管。
Abstract
Investigation of the pathophysiology of injury after intracerebral hemorrhage (ICH) requires a reproducible animal model. While ICH accounts for 10-15% of all strokes, there remains no specific effective therapy. The autologous blood injection model in mice involves the stereotaxic injection of arterial blood into the basal ganglia mimicking a spontaneous hypertensive hemorrhage in man. The response to hemorrhage can then be studied in vivo and the neurobehavioral deficits quantified, allowing for description of the ensuing pathology and the testing of potential therapeutic agents. The procedure described in this protocol uses the double injection technique to minimize risk of blood reflux up the needle track, no anticoagulants in the pumping system, and eliminates all dead space and expandable tubing in the system.
Protocol
Discussion
这在小鼠的自发性基底节出血重现模型中使用自体尾动脉的血液结果脑出血的手术模型。在小鼠的脑出血模型提供的转基因动物研究病理生理学的可用性的优势，但其体积小，使神经外科手术技术上更比在较大的动物困难。
胶原酶模型和自体血液的注入模型实验性脑出血的两个行之有效的模型。虽然胶原酶模型提供了一个简单的过程和高度重现性出血 2，使用细菌蛋?…
Divulgazioni
The authors have nothing to disclose.
Acknowledgements
这项工作是由一个转化医学和治疗学研究所的奖学金，并从医学和工程（T32HL007954）研究所的培训补助金，在美国宾夕法尼亚大学和马琳L.科恩和Jerome H. Fleisch学者格兰特在康涅狄格大学卫生中心（LHS）和国立卫生研究院NS – 029331（一汽）。
Materials
Stereotaxic frame for mouse neurosurgery (Stoelting, 51925)
Microinfusion pump and processor (UMP-3 and Micro4, World Precision Instruments, Sarasota, FL)
Mouse warmer (Stoelting, 50300)
Inhalational mouse anesthesia (Braintree Scientific, EZ-AF9000)
25 μL gastight borosilicate Hamilton syringe with coated plunger and no needle
(Hamilton company, Reno, NV, 1702RN syringe: 765401, ferrule: 30949, spacer: 30946)
fused silica needle cut to 2 cm length (Hamilton, 17739)
*note Hamilton syringe and fused silica needle may be reused for multiple surgeries if sterilized prior to each surgery. These materials are crucial to avoid blood clotting.
Sterile surgical gloves
Surgical gown, bonnet and mask
Betadine
75% ethanol
sterile 27 g needle (single use)
sterile 1 cc syringe (single use)
sterile surgical blade
Cidex
sterile water
buprenorphine and isoflurane
sterile gauze
paraffin wax paper squares
Veterinary surgical glue (Vetbond, 3M, St. Paul, MN)
Riferimenti
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