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球体的检测，以测量TGF -β诱导的入侵
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Medicina
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JoVE Journal Medicina
Spheroid Assay to Measure TGF-β-induced Invasion
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球体的检测，以测量TGF -β诱导的入侵

DOI:
10.3791/3337-v

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09:18 min

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November 16, 2011

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, , ,
1Department of Molecular Cell Biology and Centre for Biomedial Genetics,Leiden University Medical Centre

Capitoli

  • 00:05Titolo
  • 01:05Preparation of Methocel Solution
  • 01:55Spheroid Culture
  • 03:00Preparation of Collagen and Spheroid Embedding
  • 07:04Representative Results
  • 08:46Conclusion

Summary

Traduzione automatica

Traduzione automatica

描述一个分析，定量分析转化生长因子（TGF）-β诱导的三维胶原凝胶侵袭。这个实验需要的细胞株MCF10A系列，代表不同阶段乳腺癌的发展优势。通过这种方法可以用于其他细胞系，并可能被用来调查其他潜在的激活或入侵抑制剂。

Tags

Spheroid AssayMeasureTGF-βInvasionBreast Cancer ProgressionTumor SuppressorMetastasisOverexpressionPoor PrognosisMechanismsTβRIITβRI ReceptorsHeteromeric Complex FormationPhosphorylationCanonical Signaling PathwayR-SmadsSmad2Smad3Smad4Nucleus RegulationTranscription Of Target GenesNon-Smad Signaling PathwaysMAPK PathwaysMCF10A Cell SystemImmortalized MCF10A1 CellsH-RAS Transformed M1-derivative MCF10AneoT CellsPremalignant LesionsM2-derivative MCF10CA1a Cells

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