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基于荧光测定活细胞中的钙进入商店经营:从培养的肿瘤细胞,骨骼肌纤维
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber
DOI:

14:18 min

February 13, 2012

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Capitoli

  • 00:05Titolo
  • 02:11Intracellular Ca2+ Measurement for Individual Cells
  • 04:45Mn2+ Quenching Assay in Cultured Cells
  • 05:20Mn2+ Quenching Assay in Muscle Cells
  • 06:40Spatially and Temporally Resolved SOCE in Skinned Muscle Fibers
  • 10:20SOCE Activity in KYSE-150 and Muscle Cells

Summary

Traduzione automatica

店经营CA的程度<sup> 2</sup使用荧光钙可以监视>进入(SOCE)<sup> 2</sup>指标。锰<sup> 2</sup>等指标检测在培养细胞和骨骼肌纤维的SOCE淬火。允许的时间和空间分辨率SOCE机械剥皮的肌肉纤维的共焦成像的一种技术也被描述。

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