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Chapters
- 00:05Title
- 02:01Preparing Biotinylated Antigen
- 02:58Preparing Antigen Saturated Beads
- 04:08Preparing Antibody Samples and Plating the Experiment
- 05:44Flow Cytometric Analysis
- 06:29Antigen-saturated Beads and Phagocytosed in an Antibody-dependent Manner
- 08:24Conclusion
Vi presenterer en high-throughput flowcytometrisk assay å bestemme phagocytic aktiviteten av antigen-spesifikke antistoffer fra kliniske prøver, utnytte fluorescerende antigen-belagt perler og en monocyttisk cellelinje som uttrykker flere Fc reseptorer, gir reseptor bruk og phagocytic aktivitet bestemmelser i et standardisert og reproduserbar mote for enhver antigen av interesse.
Tags
Phagocytic ActivityClinical Antibody SamplesFc ReceptorsClearancePathology Of DiseaseVariable DomainsInnate Immune CellsRecombinant Monoclonal Antibody TherapyNatural InfectionVaccinationIgG SubclassGlycosylationFunctional DifferencesAntigen-specific AntibodiesDisease StateSusceptibility To InfectionProgressionClinical OutcomeEffector FunctionEnhance InfectionFc Receptor-driven PhagocytosisImmune ComplexesTh1/Th2 Polarization Assay
